Laminin-221-derived recombinant fragment facilitates isolation of cultured skeletal myoblasts.

ABSTRACT:

Introduction

Laminin is a major component of the basement membrane, containing multiple domains that bind integrin, collagen, nidogen, dystroglycan, and heparan sulfate. Laminin-221, expressed in skeletal and cardiac muscles, has strong affinity for the cell-surface receptor, integrin α7X2β1. The E8 domain of laminin-221, which is essential for cell integrin binding, is commercially available as a purified recombinant protein fragment. In this study, recombinant E8 fragment was used to purify primary rodent myoblasts. We established a facile and inexpensive method for primary myoblast culture exploiting the high affinity binding of integrin α7X2β1 to laminin-221.

Methods

Total cell populations from dissociated muscle tissue were enzymatically digested and seeded onto laminin

SUBMITTER: Kihara Y 

PROVIDER: S-EPMC9111930 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature