Micro-RNA-124-5p promotes insulin producing cell differentiation through regulating transcriptional factor NKX6.1.

ABSTRACT:

Aims

Differentiating human embryonic stem cells into pancreatic β cells has been proposed as a practical approach to managing diabetes. There have been several protocols attempting to generate β-like cells or insulin-producing cells (IPCs), but their low efficiency is a common issue. The expression level of Nkx6.1 is crucial for maintaining pancreatic β cell identity, while the proportion of PDX1 and Nkx6.1 double positive cells were not satisfied in the present protocols, leading to relative low efficiency in the differentiation into IPCs. This study aims to identify the mechanism underlying the regulation of Nkx6.1 during IPC differentiation and provide new insights for diabetes therapy.

Methods

In the current study, human embryonic stem cell (hESC) line H1 was used to per

SUBMITTER: Zhang X 

PROVIDER: S-EPMC9111988 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature