Optimization of the antimicrobial peptide Bac7 by deep mutational scanning.

ABSTRACT:

Background

Intracellularly active antimicrobial peptides are promising candidates for the development of antibiotics for human applications. However, drug development using peptides is challenging as, owing to their large size, an enormous sequence space is spanned. We built a high-throughput platform that incorporates rapid investigation of the sequence-activity relationship of peptides and enables rational optimization of their antimicrobial activity. The platform is based on deep mutational scanning of DNA-encoded peptides and employs highly parallelized bacterial self-screening coupled to next-generation sequencing as a readout for their antimicrobial activity. As a target, we used Bac7_1-23, a 23 amino acid residues long variant of bactenecin-7, a potent translationa

SUBMITTER: Koch P 

PROVIDER: S-EPMC9112550 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature