Direct bone marrow injection of human bone marrow-derived stromal cells into mouse femurs results in greater prostate cancer PC-3 cell proliferation, but not specifically proliferation within the injected femurs.

ABSTRACT:

Background

While prostate cancer (PCa) cells most often metastasize to bone in men, species-specific differences between human and mouse bone marrow mean that this pattern is not faithfully replicated in mice. Herein we evaluated the impact of partially humanizing mouse bone marrow with human bone marrow-derived stromal cells (BMSC, also known as "mesenchymal stem cells") on human PCa cell behaviour.

Methods

BMSC are key cellular constituents of marrow. We used intrafemoral injection to transplant 5 × 10⁵ luciferase (Luc) and green fluorescence protein (GFP) expressing human BMSC (hBMSC-Luc/GFP) into the right femur of non-obese diabetic (NOD)-severe combined immunodeficiency (scid) interleukin (IL)-2γ^-/- (NSG) mice. Two weeks later, 2.5 × 10⁶

SUBMITTER: Nowlan B 

PROVIDER: S-EPMC9112579 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature