Generation and application of pseudo-long reads for metagenome assembly.

ABSTRACT:

Background

Metagenomic assembly using high-throughput sequencing data is a powerful method to construct microbial genomes in environmental samples without cultivation. However, metagenomic assembly, especially when only short reads are available, is a complex and challenging task because mixed genomes of multiple microorganisms constitute the metagenome. Although long read sequencing technologies have been developed and have begun to be used for metagenomic assembly, many metagenomic studies have been performed based on short reads because the generation of long reads requires higher sequencing cost than short reads.

Results

In this study, we present a new method called PLR-GEN. It creates pseudo-long reads from metagenomic short reads based on given reference genome sequenc

SUBMITTER: Sim M 

PROVIDER: S-EPMC9112764 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature