Diabetic bladder dysfunction progresses from an overactive to an underactive phenotype in a type-1 diabetic mouse model (Akita female mouse) and is dependent on NLRP3.

ABSTRACT:

Aims

Diabetic bladder dysfunction (DBD) is a prevalent diabetic complication thought to progress from overactive (OAB) to underactive (UAB) bladder. Previously we found OAB at 15 weeks in the Akita mouse, a genetic model of Type 1 diabetes. The first aim of this study assesses bladder function at 30 weeks to assess progression. In addition, inflammation triggered by the NLRP3 inflammasome is implicated in DBD. In a second aim we assessed a role for NLRP3 by crossing Akita mice with NLRP3^-/- mice.

Main methods

Akita mice were bred with NLRP3^-/- mice. The effect of diabetes was assessed by comparing nondiabetic to diabetic mice (all NLRP3^+/+). The effect of diabetes in the absence of the NLRP3 inflammasome was assessed by comparing nondiabetic/

SUBMITTER: Hughes FM 

PROVIDER: S-EPMC9112812 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature