Regulation of Mannitol Metabolism in Enterococcus faecalis and Association with par_EF0409 Toxin-Antitoxin Locus Function.

ABSTRACT: The par_EF0409 type I toxin-antitoxin locus is situated between genes for two paralogous mannitol family phosphoenolpyruvate phosphotransferase systems (PTSs). In order to address the possibility that par_EF0409 function was associated with sugar metabolism, genetic and phenotypic analyses were performed on the flanking genes. It was found that the genes were transcribed as two operons: the downstream operon essential for mannitol transport and metabolism and the upstream operon performing a regulatory function. In addition to genes for the PTS components, the upstream operon harbors a gene similar to mtlR, the key regulator of mannitol metabolism in other Gram-positive bacteria. We confirmed that this gene is essential for the regulation of the dow

SUBMITTER: Anbalagan S 

PROVIDER: S-EPMC9112947 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature