A mesenchymal to epithelial switch in Fgf10 expression specifies an evolutionary-conserved population of ionocytes in salivary glands.

ABSTRACT: Fibroblast growth factor 10 (FGF10) is well established as a mesenchyme-derived growth factor and a critical regulator of fetal organ development in mice and humans. Using a single-cell RNA sequencing (RNA-seq) atlas of salivary gland (SG) and a tamoxifen inducible Fgf10^CreERT2:R26-tdTomato mouse, we show that FGF10^pos cells are exclusively mesenchymal until postnatal day 5 (P5) but, after P7, there is a switch in expression and only epithelial FGF10^pos cells are observed after P15. Further RNA-seq analysis of sorted mesenchymal and epithelial FGF10^pos cells shows that the epithelial FGF10^pos population express the hallmarks of ancient ionocyte signature Forkhead box i1 and 2 (Foxi1, Foxi2), Achaete-scute homolog 3 (Ascl3), and the cys

SUBMITTER: Mauduit O 

PROVIDER: S-EPMC9113928 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature