SARS-CoV-2 Permissive glioblastoma cell line for high throughput antiviral screening.

ABSTRACT: Despite the great success of the administered vaccines against SARS-CoV-2, the virus can still spread, as evidenced by the current circulation of the highly contagious Omicron variant. This emphasizes the additional need to develop effective antiviral countermeasures. In the context of early preclinical studies for antiviral assessment, robust cellular infection systems are required to screen drug libraries. In this study, we reported the implementation of a human glioblastoma cell line, stably expressing ACE2, in a SARS-CoV-2 cytopathic effect (CPE) reduction assay. These glioblastoma cells, designated as U87.ACE2⁺, expressed ACE2 and cathepsin B abundantly, but had low cellular levels of TMPRSS2 and cathepsin L. The U87.ACE2⁺ cells fused highly efficiently and quick

SUBMITTER: Vanhulle E 

PROVIDER: S-EPMC9113983 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature