Phytochemical analysis of Daphne pontica L. stems with their pro-apoptotic properties against DU-145 and LNCaP prostate cancer cells.

ABSTRACT:

Background

Daphne pontica is an endemic plant grown wild in the North part of Iran, with anticancer activities.

Objectives

This study aims to analyze the phytochemistry and screen the cytotoxic activity of new bioactive compounds against a panel of cancer cells, in addition to proapototic properties against prostate cancer cells.

Method

Purification procedure was done using repeated column chromatographies by MPLC and HPLC systems. The structures were elucidated by the NMR and exact mass spectroscopy, stereochemistry by NOESY, and absolute configuration by electronic circular dichroism (ECD) spectra. Cytotoxicity was done against DU 145, LNCaP, HeLa, MCF-7, and MDA-MB 231 cells by standard MTT assay. An annexin V/PI assay was performed to measure the type of death fol

SUBMITTER: Nikahd M 

PROVIDER: S-EPMC9114221 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature