Homology directed correction, a new pathway model for point mutation repair catalyzed by CRISPR-Cas.

ABSTRACT: Gene correction is often referred to as the gold standard for precise gene editing and while CRISPR-Cas systems continue to expand the toolbox for clinically relevant genetic repair, mechanistic hurdles still hinder widespread implementation. One of the most prominent challenges to precise CRISPR-directed point mutation repair centers on the prevalence of on-site mutagenesis, wherein insertions and deletions appear at the targeted site following correction. Here, we introduce a pathway model for Homology Directed Correction, specifically point mutation repair, which enables a foundational analysis of genetic tools and factors influencing precise gene editing. To do this, we modified an in vitro gene editing system which utilizes a cell-free extract, CRISPR-Cas RNP and donor DNA template to

SUBMITTER: Sansbury BM 

PROVIDER: S-EPMC9114366 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature