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Ultra-parallel label-free optophysiology of neural activity.

ABSTRACT: The electrical activity of neurons has a spatiotemporal footprint that spans three orders of magnitude. Traditional electrophysiology lacks the spatial throughput to image the activity of an entire neural network; besides, labeled optical imaging using voltage-sensitive dyes and tracking Ca²⁺ ion dynamics lack the versatility and speed to capture fast-spiking activity, respectively. We present a label-free optical imaging technique to image the changes to the optical path length and the local birefringence caused by neural activity, at 4,000 Hz, across a 200 × 200 μm² region, and with micron-scale spatial resolution and 300-pm displacement sensitivity using Superfast Polarization-sensitive Off-axis Full-field Optical Coherence Microscopy (SPoOF OCM). The undulations in the optical responses from mammalian neuronal activity were matched with field-potential electrophysiology measurements and validated with channel blockers. By directly tracking the widefield neural activity at millisecond timescales and micrometer resolution, SPoOF OCM provides a framework to progress from low-throughput electrophysiology to high-throughput ultra-parallel label-free optophysiology.

SUBMITTER: Iyer RR

PROVIDER: S-EPMC9114528 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Ultra-parallel label-free optophysiology of neural activity.

Iyer Rishyashring R RR Liu Yuan-Zhi YZ Renteria Carlos A CA Tibble Brian E BE Choi Honggu H Žurauskas Mantas M Boppart Stephen A SA

iScience 20220427 5

The electrical activity of neurons has a spatiotemporal footprint that spans three orders of magnitude. Traditional electrophysiology lacks the spatial throughput to image the activity of an entire neural network; besides, labeled optical imaging using voltage-sensitive dyes and tracking Ca<sup>2+</sup> ion dynamics lack the versatility and speed to capture fast-spiking activity, respectively. We present a label-free optical imaging technique to image the changes to the optical path length and t ...[more]

PMID: 35602935

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Dataset Information

Ultra-parallel label-free optophysiology of neural activity.

Publications

Ultra-parallel label-free optophysiology of neural activity.

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