PAT2 regulates vATPase assembly and lysosomal acidification in brown adipocytes.

ABSTRACT:

Objective

Brown adipocytes play a key role in maintaining body temperature as well as glucose and lipid homeostasis. However, brown adipocytes need to adapt their thermogenic activity and substrate utilization to changes in nutrient availability. Amongst the multiple factors influencing brown adipocyte activity, autophagy is an important regulatory element of thermogenic capacity and activity. Nevertheless, a specific sensing mechanism of extracellular amino acid availability linking autophagy to nutrient availability in brown adipocytes is unknown.

Methods

To characterize the role of the amino acid transporter PAT2/SLC36A2 in brown adipocytes, loss or gain of function of PAT2 were studied with respect to differentiation, subcellular localization, lysosomal activity and auto

SUBMITTER: Wang J 

PROVIDER: S-EPMC9114668 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature