Intrahepatic inflammatory IgA⁺PD-L1^high monocytes in hepatocellular carcinoma development and immunotherapy.

ABSTRACT:

Background

IgA neutralizes pathogens to prevent infection at mucosal sites. However, emerging evidence shows that IgA contributes to aggravating inflammation or dismantling antitumor immunity in human diseased liver. The aim of this study was to elucidate the roles of inflammation-induced intrahepatic inflammatory IgA⁺ monocytes in the development of hepatocellular carcinoma (HCC).

Methods

Patient cohorts including steatohepatitis cohort (n=61) and HCC cohort (n=271) were established. Patients' surgical and biopsy specimens were analyzed using immunohistochemistry. Multicolor flow cytometry was performed with a subset of patient samples. Single-cell RNA-Seq analysis was performed using Gene Expression Omnibus (GEO) datasets. Additionally, we performed in vitro dif

SUBMITTER: Sung PS 

PROVIDER: S-EPMC9114848 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature