Dataset Information

Thrombosis pathways in COVID-19 vs. influenza-associated ARDS: A targeted proteomics approach.

ABSTRACT:

Background

Pulmonary embolism (PE) occurs in one-third of critically-ill COVID-19 patients. Although prior studies identified several pathways contributing to thrombogenicity, it is unknown whether this is COVID-19-specific or also occurs in ARDS patients with another infection.

Objective

To compare pathway activity among patients having COVID-19 with PE (C19PE+), COVID-19 without PE (C19PE-), and influenza-associated ARDS (IAA) using a targeted proteomics approach.

Methods

We exploited an existing biorepository containing daily plasma samples to carefully match C19PE+ cases to C19PE- and IAA controls on mechanical ventilation duration, PEEP, FiO2, and cardiovascular-SOFA (n = 15 per group). Biomarkers representing various thrombosis pathways were measured using proximity extension- and ELISA-assays. Summed z-scores of individual biomarkers were used to represent total pathway activity.

Results

We observed no relevant between-group differences among 22 biomarkers associated with activation of endothelium, platelets, complement, coagulation, fibrinolysis or inflammation, except sIL-1RT2 and sST2, which were lower in C19PE- than IAA (log2-Foldchange -0.67, p = .022 and -1.78, p = .022, respectively). However, total pathway analysis indicated increased activation of endothelium (z-score 0.2 [-0.3-1.03] vs. 0.98 [-2.5--0.3], p = .027), platelets (1.0 [-1.3-3.0] vs. -3.3 [-4.1--0.6], p = .023) and coagulation (0.8 [-0.5-2.0] vs. -1.0 [-1.6-1.0], p = .023) in COVID-19 patients (C19PE+/C19PE- groups combined) compared to IAA.

Conclusion

We observed only minor differences between matched C19PE+, C19PE-, and IAA patients, which suggests individual biomarkers mostly reflect disease severity. However, analysis of total pathway activity suggested upregulation of some distinct processes in COVID-19 could be etiologically related to increased PE-risk.

SUBMITTER: Rademaker E

PROVIDER: S-EPMC9115133 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Publications

Thrombosis pathways in COVID-19 vs. influenza-associated ARDS: A targeted proteomics approach.

Rademaker Emma E Doorduijn Dennis J DJ Kusadasi Nuray N Maas Coen C Drylewicz Julia J Huisman Albert A Hoefer Imo E IE Bonten Marc J M MJM Derde Lennie P G LPG Rooijakkers Suzan H M SHM Cremer Olaf L OL

Journal of thrombosis and haemostasis : JTH 20220227 5

<h4>Background</h4>Pulmonary embolism (PE) occurs in one-third of critically-ill COVID-19 patients. Although prior studies identified several pathways contributing to thrombogenicity, it is unknown whether this is COVID-19-specific or also occurs in ARDS patients with another infection.<h4>Objective</h4>To compare pathway activity among patients having COVID-19 with PE (C19PE+), COVID-19 without PE (C19PE-), and influenza-associated ARDS (IAA) using a targeted proteomics approach.<h4>Methods</h4 ...[more]

PMID: 35150462

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Dataset Information

Thrombosis pathways in COVID-19 vs. influenza-associated ARDS: A targeted proteomics approach.

Background

Objective

Methods

Results

Conclusion

Publications

Thrombosis pathways in COVID-19 vs. influenza-associated ARDS: A targeted proteomics approach.

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