Microbial diversity and metaproteomic analysis of activated sludge responses to naphthalene and anthracene exposure.

ABSTRACT: The activated sludge process can effectively remove polycyclic aromatic hydrocarbons (PAHs) from wastewater via biodegradation. However, the degradable microorganisms and functional enzymes involved in this process remain unclear. In this study, we successfully employed a laboratory-scale sequential batch reactor to investigate variations in microbial community and protein expression in response to the addition of different PAHs and process time. The analysis of bacterial community structure by 454 pyrosequencing of the 16S rRNA gene indicated that bacteria from Burkholderiales order were dominant in PAHs treated sludge. Mass spectrometry performed with 2D protein profiles of all sludge samples demonstrated that most proteins exhibiting differential expression profiles during the pr

SUBMITTER: Li S 

PROVIDER: S-EPMC9116109 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature