Role of tRNA derived fragments in renal ischemia-reperfusion injury.

ABSTRACT:

Background

Ischemia-reperfusion injury (IRI) is one of the major causes of acute kidney injury (AKI). tRNA derived fragments (tRFs/tiRNAs) are groups of small noncoding RNAs derived from tRNAs. To date, the role of tRFs/tiRNAs in renal IRI has not been reported. Herein, we aimed to investigate the involvement of tRFs/tiRNAs in the occurrence and development of ischemia-reperfusion-induced AKI.

Methods

Moderate/severe renal IRI mouse models were established by bilateral renal pedicle clamping. The tRF/tiRNA profiles of healthy controls and moderate/severe IRI-stressed kidney tissues were sequenced by Illumina NextSeq 500. Candidate differentially expressed tiRNAs were further verified by RT-qPCR. Biological analysis was also performed.

Results

Overall, 152 tRFs/tiRNAs

SUBMITTER: Li D 

PROVIDER: S-EPMC9116270 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature