Phenotypic and Genotypic Characteristics of a Tigecycline-Resistant Acinetobacter pittii Isolate Carrying bla _NDM-1 and the Novel bla _OXA Allelic Variant bla _OXA-1045.

ABSTRACT: A tigecycline-resistant Acinetobacter pittii clinical strain from pleural fluid carrying a bla _NDM-1 gene and a novel bla _OXA gene, bla _OXA-1045, was isolated and characterized. The AP2044 strain acquired two copies of the bla _NDM-1 gene and six antibiotic resistance genes (ARGs) from other pathogens. According to the whole-genome investigation, the GC ratios of ARGs (50-60%) were greater than those of the chromosomal backbone (39.46%), indicating that ARGs were horizontally transferred. OXA-1045 belonged to the OXA-213 subfamily and the amino acid sequence of OXA-1045 showed 89% similarity to the amino acid sequences of OXA-213. Then, bla _OXA-1045 and bla _OXA-213 were cloned

SUBMITTER: Ding Z 

PROVIDER: S-EPMC9116503 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature