Gene regulation by a protein translation factor at the single-cell level.

ABSTRACT: Gene expression is inherently stochastic and pervasively regulated. While substantial work combining theory and experiments has been carried out to study how noise propagates through transcriptional regulations, the stochastic behavior of genes regulated at the level of translation is poorly understood. Here, we engineered a synthetic genetic system in which a target gene is down-regulated by a protein translation factor, which in turn is regulated transcriptionally. By monitoring both the expression of the regulator and the regulated gene at the single-cell level, we quantified the stochasticity of the system. We found that with a protein translation factor a tight repression can be achieved in single cells, noise propagation from gene to gene is buffered, and the regulated gene is sensit

SUBMITTER: Dolcemascolo R 

PROVIDER: S-EPMC9116677 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature