Hypoxia-reprogrammed regulatory group 2 innate lymphoid cells promote immunosuppression in pancreatic cancer.

ABSTRACT:

Background

Previously, we uncovered a patient subgroup with highly malignant pancreatic cancer with serum markers CEA⁺/CA125⁺/CA19-9 ≥ 1000 U/mL (triple-positive, TP). However, the underlying immunosuppressive mechanism in the tumor immune microenvironment (TIME) of this subgroup is still unknown.

Methods

Human tissues were analyzed by flow cytometry, mass cytometry, and immunofluorescence staining. Mouse pancreatic ILC2s were expanded in vivo and used for RNA sequencing, chromatin immunoprecipitation (ChIP), and chemotaxis assays.

Findings

Through microarray data, we identified the accumulation of the hypoxia-induced factor-1α (HIF-1α) pathway in these TP patients. Via flow and mass cytometry, we discovered that a special subset of ILC2s were hi

SUBMITTER: Ye L 

PROVIDER: S-EPMC9117270 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature