Identification of IQGAP1 as a SLC26A4 (Pendrin)-Binding Protein in the Kidney.

ABSTRACT: Background: Several members of the SLC26A family of transporters, including SLC26A3 (DRA), SLC26A5 (prestin), SLC26A6 (PAT-1; CFEX) and SLC26A9, form multi-protein complexes with a number of molecules (e.g., cytoskeletal proteins, anchoring or adaptor proteins, cystic fibrosis transmembrane conductance regulator, and protein kinases). These interactions provide regulatory signals for these molecules. However, the identity of proteins that interact with the Cl^-/HCO₃ ^- exchanger, SLC26A4 (pendrin), have yet to be determined. The purpose of this study is to identify the protein(s) that interact with pendrin. Methods: A yeast two hybrid (Y2H) system was employed to screen a mouse kidney cDNA library using the C-terminal fragment of SLC26A4 as bait

SUBMITTER: Xu J 

PROVIDER: S-EPMC9117723 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature