The Host-Microbiome Response to Hyperbaric Oxygen Therapy in Ulcerative Colitis Patients.

ABSTRACT:

Background & aims

Hyperbaric oxygen therapy (HBOT) is a promising treatment for moderate-to-severe ulcerative colitis. However, our current understanding of the host and microbial response to HBOT remains unclear. This study examined the molecular mechanisms underpinning HBOT using a multi-omic strategy.

Methods

Pre- and post-intervention mucosal biopsies, tissue, and fecal samples were collected from HBOT phase 2 clinical trials. Biopsies and fecal samples were subjected to shotgun metaproteomics, metabolomics, 16s rRNA sequencing, and metagenomics. Tissue was subjected to bulk RNA sequencing and digital spatial profiling (DSP) for single-cell RNA and protein analysis, and immunohistochemistry was performed. Fecal samples were also used for colonization experiments in IL10<

SUBMITTER: Gonzalez CG 

PROVIDER: S-EPMC9117812 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature