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A MACS protocol for purification of untouched germinal center B cells from unimmunized or germinal center-induced mice.

ABSTRACT: Highly enriched germinal center (GC) B cell populations are essential for studying humoral immunity. Current MACS protocols that isolate untouched GC B cells require GC induction and typically require further FACS purification with direct antibody labeling to achieve sufficiently high purities. We present a MACS protocol with progressive and repeated negative selections that yields highly purified untouched GC B cells from both unimmunized and GC-induced mice and allows further FACS isolation of unlabeled GC B cells from remaining debris by scatter.

SUBMITTER: Desikan SA

PROVIDER: S-EPMC9117920 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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A MACS protocol for purification of untouched germinal center B cells from unimmunized or germinal center-induced mice.

Desikan Shreya A SA Chavan Sailee S Ou Peiqi P Roman Christopher A J CAJ Huan Chongmin C

STAR protocols 20220514 2

Highly enriched germinal center (GC) B cell populations are essential for studying humoral immunity. Current MACS protocols that isolate untouched GC B cells require GC induction and typically require further FACS purification with direct antibody labeling to achieve sufficiently high purities. We present a MACS protocol with progressive and repeated negative selections that yields highly purified untouched GC B cells from both unimmunized and GC-induced mice and allows further FACS isolation of ...[more]

PMID: 35600926

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