Cancer cell viscoelasticity measurement by quantitative phase and flow stress induction.

ABSTRACT: Cell viscoelastic properties are affected by the cell cycle, differentiation, and pathological processes such as malignant transformation. Therefore, evaluation of the mechanical properties of the cells proved to be an approach to obtaining information on the functional state of the cells. Most of the currently used methods for cell mechanophenotyping are limited by low robustness or the need for highly expert operation. In this paper, the system and method for viscoelasticity measurement using shear stress induction by fluid flow is described and tested. Quantitative phase imaging (QPI) is used for image acquisition because this technique enables one to quantify optical path length delays introduced by the sample, thus providing a label-free objective measure of morphology and dynamics. V

SUBMITTER: Vicar T 

PROVIDER: S-EPMC9117928 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature