Kinetics and thermodynamics of BI-BII interconversion altered by T:G mismatches in DNA.

ABSTRACT: T:G mismatches in DNA result in humans primarily from deamination of methylated CpG sites. They are repaired by redundant systems, such as thymine DNA glycosylase (TDG) and methyl-binding domain enzyme (MBD4), and maintenance of these sites has been implicated in epigenetic processes. The process by which these enzymes identify a canonical DNA base in the incorrect basepairing context remains a mystery. However, the conserved contacts of the repair enzymes with the DNA backbone suggests a role for protein-phosphate interaction in the recognition and repair processes. We have used ³¹P NMR to investigate the energetics of DNA backbone BI-BII interconversion, and for this work have focused on alterations to the activation barriers to interconversion and the effect of a mismatch com

SUBMITTER: Westwood MN 

PROVIDER: S-EPMC9117933 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature