Computational epitope binning reveals functional equivalence of sequence-divergent paratopes.

ABSTRACT: The therapeutic efficacy of a protein binder largely depends on two factors: its binding site and its binding affinity. Advances in in vitro library display screening and next-generation sequencing have enabled accelerated development of strong binders, yet identifying their binding sites still remains a major challenge. The differentiation, or "binning", of binders into different groups that recognize distinct binding sites on their target is a promising approach that facilitates high-throughput screening of binders that may show different biological activity. Here we study the extent to which the information contained in the amino acid sequences comprising a set of target-specific binders can be leveraged to bin them, inferring functional equivalence of their binding regions, or p

SUBMITTER: Mahita J 

PROVIDER: S-EPMC9118127 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature