Proteomic Characterization of Senescent Laryngeal Adductor and Plantaris Hindlimb Muscles.

ABSTRACT:

Objectives

The goals of this study were to 1) compare global protein expression in muscles of the larynx and hindlimb and 2) investigate differences in protein expression between aged and nonaged muscle using label-free global proteomic profiling methods.

Methods

Liquid chromatography-mass spectrometry (LC-MS/MS) analysis was performed on thyroarytenoid intrinsic laryngeal muscle and plantaris hindlimb muscle from 10 F344xBN F1 male rats (5 old and 5 young). Protein expression was compared and pathway enrichment analysis performed for each muscle type (larynx and limb) and age group (old and young muscle).

Results

Over 1,000 proteins were identified in common across both muscle types and age groups using LC-MS/MS analysis. Significant age-related differences were seen

SUBMITTER: Shembel AC 

PROVIDER: S-EPMC9118136 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature