Chromatin remodeling is restricted by transient GATA6 binding during iPSC differentiation to definitive endoderm.

ABSTRACT: In addition to cooperatively driving transcriptional programs, emerging evidence supports transcription factors interacting with one another to modulate the outcome of binding events. As such, transcription factor interactions fine-tune the unique gene expression profiles required for developmental progression. Using human-induced pluripotent stem cells as a model of human endoderm lineage commitment, we reveal that GATA6 transiently co-localizes with EOMES at regions associated with non-endodermal lineages and is required for the repression of chromatin opening at these loci. Our results indicate that GATA6-dependent repression of chromatin remodeling, which is potentially mediated via the recruitment of NCOR1 to the EOMES interactome, contributes to definitive endoderm commitment. We ant

SUBMITTER: Heslop JA 

PROVIDER: S-EPMC9118154 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature