DNA-Binding Properties of Bis-N-substituted Tetrandrine Derivatives.

ABSTRACT: A series of bis-N-substituted tetrandrine derivatives carrying different aromatic substituents attached to both nitrogen atoms of the natural alkaloid were studied with double-stranded model DNAs (dsDNAs) to examine the binding properties and mechanism. Variable-temperature molecular recognition studies using UV-vis and fluorescence techniques revealed the thermodynamic parameters, ΔH, ΔS, and ΔG, showing that the tetrandrine derivatives exhibit high affinity toward dsDNA (K ≈ 10⁵-10⁷ M^-1), particularly the bis(methyl)anthraquinone (BAqT) and bis(ethyl)indole compounds (BInT). Viscometry experiments, ethidium displacement assays, and molecular modeling studies enabled elucidation of the possible binding mode, indicating th

SUBMITTER: Gonzalez-Martinez SM 

PROVIDER: S-EPMC9118212 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature