Revealing the novel complexity of plant long non-coding RNA by strand-specific and whole transcriptome sequencing for evolutionarily representative plant species.

ABSTRACT:

Background

Previous studies on plant long noncoding RNAs (lncRNAs) lacked consistency and suffered from many factors like heterogeneous data sources and experimental protocols, different plant tissues, inconsistent bioinformatics pipelines, etc. For example, the sequencing of RNAs with poly(A) tails excluded a large portion of lncRNAs without poly(A), and use of regular RNA-sequencing technique did not distinguish transcripts' direction for lncRNAs. The current study was designed to systematically discover and analyze lncRNAs across eight evolutionarily representative plant species, using strand-specific (directional) and whole transcriptome sequencing (RiboMinus) technique.

Results

A total of 39,945 lncRNAs (25,350 lincRNAs and 14,595 lncNATs) were identified, which showed

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC9118565 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature