A protocol for Chenopodium quinoa pollen germination.

ABSTRACT:

Background

Quinoa is an increasingly popular seed crop frequently studied for its tolerance to various abiotic stresses as well as its susceptibility to heat. Estimations of quinoa pollen viability through staining methods have resulted in conflicting results. A more effective alternative to stains is to estimate pollen viability through in vitro germination. Here we report a method for in vitro quinoa pollen germination that could be used to understand the impact of various stresses on quinoa fertility and therefore seed yield or to identify male-sterile lines for breeding.

Results

A semi-automated method to count germinating pollen was developed in PlantCV, which can be widely used by the community. Pollen collected on day 4 after first anthesis at zeitgeber time 5 was opt

SUBMITTER: Castillo SE 

PROVIDER: S-EPMC9118578 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature