Contribution of a novel gene to lysergic acid amide synthesis in Metarhizium brunneum.

ABSTRACT:

Objective

The fungus Metarhizium brunneum produces ergot alkaloids of the lysergic acid amide class, most abundantly lysergic acid α-hydroxyethylamide (LAH). Genes for making ergot alkaloids are clustered in the genomes of producers. Gene clusters of LAH-producing fungi contain an α/β hydrolase fold protein-encoding gene named easP whose presence correlates with LAH production but whose contribution to LAH synthesis in unknown. We tested whether EasP contributes to LAH accumulation through gene knockout studies.

Results

We knocked out easP in M. brunneum via a CRISPR/Cas9-based approach, and accumulation of LAH was reduced to less than half the amount observed in the wild type. Because LAH accumulation was reduced and not eliminated, we identified and mutated the only close

SUBMITTER: Britton KN 

PROVIDER: S-EPMC9118626 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature