Determination of protoplast growth properties using quantitative single-cell tracking analysis.

ABSTRACT:

Background

Although quantitative single-cell analysis is frequently applied in animal systems, e.g. to identify novel drugs, similar applications on plant single cells are largely missing. We have exploited the applicability of high-throughput microscopic image analysis on plant single cells using tobacco leaf protoplasts, cell-wall free single cells isolated by lytic digestion. Protoplasts regenerate their cell wall within several days after isolation and have the potential to expand and proliferate, generating microcalli and finally whole plants after the application of suitable regeneration conditions.

Results

High-throughput automated microscopy coupled with the development of image processing pipelines allowed to quantify various developmental properties of thousands of

SUBMITTER: Dawson J 

PROVIDER: S-EPMC9118701 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature