Using unique ORFan genes as strain-specific identifiers for Escherichia coli.

ABSTRACT:

Background

Bacterial identification at the strain level is a much-needed, but arduous and challenging task. This study aimed to develop a method for identifying and differentiating individual strains among multiple strains of the same bacterial species. The set used for testing the method consisted of 17 Escherichia coli strains picked from a collection of strains isolated in Germany, Spain, the United Kingdom and Vietnam from humans, cattle, swine, wild boars, and chickens. We targeted unique or rare ORFan genes to address the problem of selective and specific strain identification. These ORFan genes, exclusive to each strain, served as templates for developing strain-specific primers.

Results

Most of the experimental strains (14 out of 17) possessed unique ORFan genes that

SUBMITTER: Ferrandis-Vila M 

PROVIDER: S-EPMC9118744 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature