Rapid molecular diagnostics of tuberculosis resistance by targeted stool sequencing.

ABSTRACT:

Background

Stool is an important diagnostic specimen for tuberculosis in populations who struggle to provide sputum, such as children or people living with HIV. However, the culture of Mycobacterium tuberculosis (M. tuberculosis) complex strains from stool perform poorly. This limits the opportunity for phenotypic drug resistance testing with this specimen. Therefore, reliable molecular methods are urgently needed for comprehensive drug resistance testing on stool specimens.

Methods

We evaluated the performance of targeted next-generation sequencing (tNGS, Deeplex® Myc-TB) for the detection of mutations associated with M. tuberculosis complex drug resistance on DNA isolated from stool specimens provided by participants from a prospective cohort of patients treated for tuberc

SUBMITTER: Sibandze DB 

PROVIDER: S-EPMC9118838 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature