Directed evolution of Zymomonas mobilis sugar facilitator Glf to overcome glucose inhibition.

ABSTRACT: Cellular import of D-xylose, the second most abundant sugar in typical lignocellulosic biomass, has been evidenced to be an energy-depriving process in bacterial biocatalysts. The sugar facilitator of Zymomonas mobilis, Glf, is capable of importing xylose at high rates without extra energy input, but is inhibited by D-glucose (the primary biomass sugar), potentially limiting the utility of this transporter for fermentation of sugar mixtures derived from lignocellulose. In this work we developed an Escherichia coli platform strain deficient in glucose and xylose transport to facilitate directed evolution of Glf to overcome glucose inhibition. Using this platform, we isolated nine Glf variants created by both random and site-saturation mutagenesis with increased xylose utilization rates rang

SUBMITTER: Kurgan G 

PROVIDER: S-EPMC9118996 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature