RPS12 and UBC4 Are Related to Senescence Signal Production in the Ribosomal RNA Gene Cluster.

ABSTRACT: Genome instability causes cellular senescence in many organisms. The rRNA gene cluster (rDNA) is one of the most unstable regions in the genome and this instability might convey a signal that induces senescence in the budding yeast. The instability of rDNA mostly depends on replication fork blocking (RFB) activity which induces recombination and gene amplification. By overexpression of Fob1, responsible for the RFB activity, we found that unstable rDNA induces cell cycle arrest and restricts replicative life span. We isolated yeast mutants that grew normally while Fob1 was overexpressed, expecting that some of the mutated genes would be related to the production of a "senescence signal" that elongates cell cycle, stops cell division and finally restricts replicative life span. Our screen i

SUBMITTER: Yanagi S 

PROVIDER: S-EPMC9119118 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature