Building a Simple and Versatile Illumination System for Optogenetic Experiments.

ABSTRACT: Controlling biological processes using light has increased the accuracy and speed with which researchers can manipulate many biological processes. Optical control allows for an unprecedented ability to dissect function and holds the potential for enabling novel genetic therapies. However, optogenetic experiments require adequate light sources with spatial, temporal, or intensity control, often a bottleneck for researchers. Here we detail how to build a low-cost and versatile LED illumination system that is easily customizable for different available optogenetic tools. This system is configurable for manual or computer control with adjustable LED intensity. We provide an illustrated step-by-step guide for building the circuit, making it computer-controlled, and constructing the LEDs. To fac

SUBMITTER: Kyriakakis P 

PROVIDER: S-EPMC9119341 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature