Nano-hydroxyapatite improves intestinal absorption of acetazolamide (BCS Class IV drug)-but how?

ABSTRACT: We earlier reported that coating poorly water-soluble drugs with nano-hydroxyapatite (nano-HAP) improves bioavailability after oral administration. In the present study, we coated BCS Class IV drug acetazolamide (AZ) with nano-HAP (AZ/HAP formulation), and investigated its bioavailability and nano-HAP's role in promoting it. We tested AZ bioavailability after a single oral dose of the AZ/HAP formulation in rats, followed by a series of in vitro, ex vivo and in vivo testing. The binding state of AZ and nano-HAP was analyzed by gel filtration chromatography. AZ permeability was studied using a Caco-2 cell monolayer assay kit, to test for tight junction penetration, then using an Ussing chamber mounted with intestinal epithelium, both with and without Peyer's patch tissue, to examine the role

SUBMITTER: Kaneko K 

PROVIDER: S-EPMC9119549 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature