RecombineX: A generalized computational framework for automatic high-throughput gamete genotyping and tetrad-based recombination analysis.

ABSTRACT: Meiotic recombination is an essential biological process that ensures faithful chromosome segregation and promotes parental allele shuffling. Tetrad analysis is a powerful approach to quantify the genetic makeups and recombination landscapes of meiotic products. Here we present RecombineX (https://github.com/yjx1217/RecombineX), a generalized computational framework that automates the full workflow of marker identification, gamete genotyping, and tetrad-based recombination profiling based on any organism or genetic background with batch processing capability. Aside from conventional reference-based analysis, RecombineX can also perform analysis based on parental genome assemblies, which facilitates analyzing meiotic recombination landscapes in their native genomic contexts. Additional feat

SUBMITTER: Li J 

PROVIDER: S-EPMC9119626 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature