LDL associates with pro-inflammatory monocyte subset differentiation and increases in chemokine receptor profile expression in African Americans.

ABSTRACT:

Background

In the United States, African Americans (AAs) have greater risk for Class III obesity and cardiovascular disease (CVD). Previous reports suggest that AAs have a different immune cell profile when compared to Caucasians.

Methods

The immune cell profile of AAs was characterized by flow cytometry using two experimental setups: ex vivo (N = 40) and in vitro (N = 10). For ex vivo experiments, PBMC were treated with participant serum to understand how lipid contents may contribute to monocyte phenotypic differences. For in vitro experiments, monocytes were low-density lipoprotein (LDL)- or vehicle-treated for four hours and subsequently analyzed by flow cytometry and RT-qPCR.

Results

When PBMCs were treated with participant sera, subsequent multivariable regressi

SUBMITTER: Gutierrez-Huerta CA 

PROVIDER: S-EPMC9120048 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature