Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics.

ABSTRACT: Mammalian splenic tissue is rich in functional immune cells, primarily lymphocytes which can mask low-abundance populations in downstream analyses. This protocol enriches minority immune cell populations from mouse spleen via immunomagnetic negative depletion to generate an untouched enriched cell fraction. Enriched cells are then spiked with untouched splenocytes in a controlled repopulation, validated by flow cytometry and results in a single-cell transcriptomic clustering analysis with a broadened cellular landscape.

SUBMITTER: Schulze TT 

PROVIDER: S-EPMC9120244 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature