Automated characterisation of neutrophil activation phenotypes in ex vivo human Candida blood infections.

ABSTRACT: Rapid identification of pathogens is required for early diagnosis and treatment of life-threatening bloodstream infections in humans. This requirement is driving the current developments of molecular diagnostic tools identifying pathogens from human whole blood after successful isolation and cultivation. An alternative approach is to determine pathogen-specific signatures from human host immune cells that have been exposed to pathogens. We hypothesise that activated immune cells, such as neutrophils, may exhibit a characteristic behaviour - for instance in terms of their speed, dynamic cell morphology - that allows (i) identifying the type of pathogen indirectly and (ii) providing information on therapeutic efficacy. In this feasibility study, we propose a method for the quantitative asses

SUBMITTER: Belyaev I 

PROVIDER: S-EPMC9120255 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature