Survival of Stem Cells and Progenitors in the Intestine Is Regulated by LPA₅-Dependent Signaling.

ABSTRACT:

Background & aims

Regeneration of the epithelium by stem cells in the intestine is supported by intrinsic and extrinsic factors. Lysophosphatidic acid (LPA), a bioactive lipid mediator, regulates many cellular functions, including cell proliferation, survival, and cytokine secretion. Here, we identify LPA₅ receptor as a potent regulator of the survival of stem cells and transit-amplifying cells in the intestine.

Methods

We have used genetic mouse models of conditional deletion of Lpar5, Lpar5^f/f;Rosa-Cre^ERT (Lpar5^KO), and intestinal epithelial cell-specific Lpar5^f/f;AhCre (Lpar5^IECKO) mice. Mice were treated with tamoxifen or β-naphthoflavone to delete Lpar5 expression. Enteroids derived from these mice were u

SUBMITTER: Liang Z 

PROVIDER: S-EPMC9120264 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature