The standardisation of the approach to metagenomic human gut analysis: from sample collection to microbiome profiling.

ABSTRACT: In recent years, the number of metagenomic studies increased significantly. Wide range of factors, including the tremendous community complexity and variability, is contributing to the challenge in reliable microbiome community profiling. Many approaches have been proposed to overcome these problems making hardly possible to compare results of different studies. The significant differences between procedures used in metagenomic research are reflected in a variation of the obtained results. This calls for the need for standardisation of the procedure, to reduce the confounding factors originating from DNA isolation, sequencing and bioinformatics analyses in order to ensure that the differences in microbiome composition are of a true biological origin. Although the best practices for metagen

SUBMITTER: Szostak N 

PROVIDER: S-EPMC9120454 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature