Identification of anti-adipogenic withanolides from the roots of Indian ginseng (Withania somnifera).

ABSTRACT:

Background

Withania somnifera (Solanaceae), generally known as Indian ginseng, is a medicinal plant that is used in Ayurvedic practice for promoting health and longevity. This study aims to identify the bioactive metabolites from Indian ginseng and elucidate their structures.

Methods

Withanolides were purified by chromatographic techniques, including HPLC coupled with LC/MS. Chemical structures of isolated withanolides were clarified by analyzing the spectroscopic data from 1D and 2D NMR, and HR-ESIMS experiment. Absolute configurations of the withanolides were established by the application of NMR chemical shifts and ECD calculations. Anti-adipogenic activities of isolates were evaluated using 3T3-L1 preadipocytes with Oil Red O staining and quantitative real-time PC

SUBMITTER: Lee SR 

PROVIDER: S-EPMC9120796 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature