Exploring the underlying molecular mechanism of liver cancer cells under hypoxia based on RNA sequencing.

ABSTRACT:

Background

The aim of our study was to use the differentially expressed mRNAs (DEmRNAs) and differentially expressed miRNAs (DEmiRNAs) to illustrate the underlying mechanism of hypoxia in liver cancer.

Methods

In this study, a cell model of hypoxia was established, and autophagy activity was measured with western blotting and transmission electron microscopy. The effect of hypoxia conditions on the invasion of liver cancer cell was evaluated. RNA sequencing was used to identify DEmRNAs and DEmiRNAs to explore the mechanism of hypoxia in liver cancer cells.

Results

We found that autophagy activation was triggered by hypoxia stress and hypoxia might promote liver cancer cell invasion. In addition, a total of 407 shared DEmRNAs and 57 shared DEmiRNAs were identified in b

SUBMITTER: Zhao X 

PROVIDER: S-EPMC9121577 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature