T Cell Epitope Discovery in the Context of Distinct and Unique Indigenous HLA Profiles.

ABSTRACT: CD8⁺ T cells are a pivotal part of the immune response to viruses, playing a key role in disease outcome and providing long-lasting immunity to conserved pathogen epitopes. Understanding CD8⁺ T cell immunity in humans is complex due to CD8⁺ T cell restriction by highly polymorphic Human Leukocyte Antigen (HLA) proteins, requiring T cell epitopes to be defined for different HLA allotypes across different ethnicities. Here we evaluate strategies that have been developed to facilitate epitope identification and study immunogenic T cell responses. We describe an immunopeptidomics approach to sequence HLA-bound peptides presented on virus-infected cells by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Using antigen presenting cell lines that st

SUBMITTER: Hensen L 

PROVIDER: S-EPMC9121770 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature