Reovirus μ2 protein modulates host cell alternative splicing by reducing protein levels of U5 snRNP core components.

ABSTRACT: Mammalian orthoreovirus (MRV) is a double-stranded RNA virus from the Reoviridae family presenting a promising activity as an oncolytic virus. Recent studies have underlined MRV's ability to alter cellular alternative splicing (AS) during infection, with a limited understanding of the mechanisms at play. In this study, we investigated how MRV modulates AS. Using a combination of cell biology and reverse genetics experiments, we demonstrated that the M1 gene segment, encoding the μ2 protein, is the primary determinant of MRV's ability to alter AS, and that the amino acid at position 208 in μ2 is critical to induce these changes. Moreover, we showed that the expression of μ2 by itself is sufficient to trigger AS changes, and its ability to enter the nucleus is not required for all these chan

SUBMITTER: Boudreault S 

PROVIDER: S-EPMC9122528 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature