Proteome allocations change linearly with the specific growth rate of Saccharomyces cerevisiae under glucose limitation.

ABSTRACT: Saccharomyces cerevisiae is a widely used cell factory; therefore, it is important to understand how it organizes key functional parts when cultured under different conditions. Here, we perform a multiomics analysis of S. cerevisiae by culturing the strain with a wide range of specific growth rates using glucose as the sole limiting nutrient. Under these different conditions, we measure the absolute transcriptome, the absolute proteome, the phosphoproteome, and the metabolome. Most functional protein groups show a linear dependence on the specific growth rate. Proteins engaged in translation show a perfect linear increase with the specific growth rate, while glycolysis and chaperone proteins show a linear decrease under respiratory conditions. Glycolytic enzymes and chaperones, however, sh

SUBMITTER: Xia J 

PROVIDER: S-EPMC9122918 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature